Royal Free London NHS Foundation Trust
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Haemophilia and thrombosis: the laboratory

The haemophilia centre and thrombosis unit provides comprehensive laboratory services for the diagnosis and management of various inherited and acquired bleeding and thrombotic disorders. The laboratory is located on the first floor of the medical school at the Royal Free London NHS Foundation Trust.

Clinical Pathology Accreditation UK reference no. 1063The laboratories have been fully accredited by Clinical Pathology Accreditation (CPA) UK Ltd since 1994. The routine and specialist diagnostic service provides a range of tests for the diagnosis of various inherited and acquired bleeding and thrombotic disorders.

The service includes:
  • A highly specialised phenotypic service for the definitive diagnosis of congenital bleeding and thrombotic disorders
  • Diagnosis and treatment monitoring of patients registered at the Haemophilia Centre & Thrombosis Unit
  • A molecular genetic laboratory service in inherited bleeding disorders
  • A molecular genetic laboratory service for the screening of abnormalities in thrombophilia
  • An out of hours service to cover specialised haemostasis and thrombotic tests
  • A laboratory reference service for other haemophilia treatment units including assistant with supplies of reagents and standards when necessary
  • A national educational service for clinicians, clinical scientists, biomedical scientists and medical personnel invited to the department to receive training through the International Haemophilia Training Centre (IHTC) programmes and fellowship placements.
  • A satellite routine coagulation service to the Pathology Laboratory at the Royal National Orthopaedic Hospital in Stanmore

There is an out of hours on call specialist service provided by a haemostasis biomedical scientist which can be arranged by discussion with the consultant on call for RFL HC & TU through switchboard.

Our test repertoire includes: test finder >>

The following key factors are known to affect the performance of a test
  • Under/overfilled/out of date citrate tubes
  • Clotted samples
  • Haemolysed samples
  • Heparin contaminated samples
  • Samples for coagulation tests which are greater than four hours old
  • Samples for INR which are greater than six hours old

Additional requests for routine haemostasis tests

Please call the laboratory during routine hours for additional tests, as these can only be added onto samples, within the department, which are taken on the same day and within the specified timescale stated above.

The repertoire of additional tests includes:
  • D-Dimer
  • Fibrinogen
  • Thrombin Time
  • Reptilase Time

Additional request for specialist haemostasis tests

Please note that for further specialist haemostasis tests, extra samples are required.

Routine screening and specialist laboratory service is available from 9am to 5.30pm Monday to Friday

Routine screening and specialist laboratory service is available from 9am to 5.30pm Monday to Friday

Weekends and pubic holidays the laboratory service is available from 9am to 5pm

All out of hours services are provided by the RFL haematology/blood transfusion department (5pm–9am Monday - Sunday.

Sample requirements

Please contact us as the requirements will depend on the tests requested. Please see the test finder >>


Laboratory reception 020 7794 0500 ext 34207, 34485
Laboratory manager, Anne Riddell 020 7830 2274 or ext 34484
Molecular genetics laboratory, Gillian Mellars 020 7472 6412 or ext 35994
Quality manager, Mary-Anne Pickering 020 7794 0500 or ext 35985

Address for sending samples

Anne Riddell
Laboratory manager
Katharine Dormandy Haemophilia Centre & Thrombosis Unit Laboratory
Royal Free London NHS Foundation Trust
Pond Street
London NW3 2QG

Routine diagnostic testing

  • Coagulation screening tests – PT/INR, APTT, Thrombin Time, Clauss Fibrinogen, Reptilase Time, D-Dimers
  • Anti Xa for heparin monitoring

Bleeding disorders workup

  • Coagulation factor assays, Inhibitor screening and Bethesda/Nijmegen assays von Willebrand Factor (VWF) assays - VWF: Ag, VWF: CB, VWF: RCO, VWF:Multimers, VWF: FVIII binding assay, Platelet RIPA
  • Platelet aggregation, Platelet nucleotide assay, PFA-100™, IMPACT-R™
  • Plasmin inhibitor

Thrombotic workup

  • Protein C: activity and antigen
  • Protein S: total, free and activity
  • Antithrombin: antigen and activity
  • Lupus Anticoagulant
  • Plasminiogen
  • PAI-1: antigen
  • Factor V Leiden
  • Prothrombin G20210A variant
  • Novel Anticoagulant Monitoring

Other assays

  • Anti PF4 (HIT screening)
  • Thrombin Antithrombin assays (TAT)
  • Prothrombin Fragments 1+2
  • Fibrinogen antigen
  • VWF:pro-peptide assays

Research tests

The laboratory actively participates in research related to patients with various bleeding and thrombotic problems:

  • Thrombin Generation Assays
  • Thromboelastography (ROTEM & TEG)

page last reviewed: 08 January 2013